AnaSpec Introduces HTS-Optimized EnzoLyte™ Caspase Assay Kits

These range from normal fetal development to diseases, such as cancer2, organ failure and neurodegenerative diseases. During apoptosis, caspases execute the disassembly of cellular components by proteolytic cleavage of a variety of substrates, such as poly-(ADP ribose) polymerase3, DNA-dependent protein kinase (DNA-PK), topoisomerases, and protein kinase C delta4. At least ten caspases have been discovered. Some of these caspases identify and cleave a specific peptide substrate, while others recognize the same peptide substrate4.

AnaSpec’s line of EnzoLyte™ Caspase Assay Kits are optimized for high-throughput screening. HTS-optimized features include: one-step homogeneous assays, a convenient mix-and-read format, and highly sensitive fluorimetric components.

EnzoLyte™ Homogeneous Caspase-3/7 Assay Kits
- For detecting caspase 3/7 activity in cells and/or for screening caspase 3/7 inducers or inhibitors
- Measures activity directly in 96- or 384-well microplate, bifunctional lysis buffer lyses cells as well as provides optimal buffer condition for caspase 3/7 activity

EnzoLyte™ Homogeneous AFC Caspase-3/7 Assay Kit
· 500 assays in 96-well format
· Ex/Em(nm)=370/500

EnzoLyte™ Homogeneous AMC Caspase-3/7 Assay Kit
· 500 assays in 96-well format
· Ex/Em(nm)=340/440

EnzoLyte™ Homogeneous Rh110 Caspase-3/7 Assay Kit
· 500 assays in 96-well format
· Ex/Em(nm)=501/527

EnzoLyte™ Caspase Profiling Kits
- For assaying caspase protease activities

EnzoLyte™ AMC Caspase Profiling Kit
· 8 AMC-based substrates coated to two 96-well plates, AMC fluorescence reference std coated on the same plate
· Ex/Em=340/440 nm
EnzoLyte™ AFC Caspase Profiling Kit
· 8 AFC-based substrates coated to two 96-well plates, AFC fluorescence reference std coated on same plate
· Ex/Em=370/500 nm
EnzoLyte™ Caspase Substrate Sampler Kits
- The best solution for identifying a suitable substrate for designing AFC-based caspase assays

EnzoLyte™ AMC Caspase Substrate Sampler Kit
· 8 AMC-based substrates in individual vials. Each substrate contains ample amount for 100 assays in a 96-well format
· Ex/Em=340/440 nm
EnzoLyte™ AFC Caspase Substrate Sampler Kit
· 8 AFC-based substrates in individual vials. Each substrate contains ample amount for 100 assays in a 96-well format
· Ex/Em=370/500 nm

For more information, visit www.anaspec.com

References
1. Thornberry, NA. and Y. Lazebnik, Science 281, 1312-1316 (1998).
2. Reed, JC. J. Clin. Oncol. 17, 2941-2953 (1999).
3. Lazebnik, YA. et al. Nature 371, 346-347 (1994).
4. Villa, P. et al. Trends Biochem. Sci. 22, 388-393 (1997).