Trevigen, Inc. Releases HA-R-Spondin1-Fc 293T Cell Line for the Propagation of Organoid Cultures
January 09, 2016 (PRLEAP.COM) Health NewsJanuary 9, 2016 - R-Spondin1 proteins positively regulate Wnt/beta-catenin signaling, most likely by acting as a ligand for LGR4-6 receptors and an inhibitor for ZNRF3. R-Spondin-1 induces proliferation of intestinal crypt epithelial cells, increases intestinal epithelial healing, and supports intestinal epithelial stem cell renewal.
The Trevigen 293T cell line is stably transfected to express murine Rspo1 with an N-terminal HA epitope tag and fused to a C-terminal murine IgG2a Fc fragment. This cell line is used to produce either purified Rspo1 or Rspo1 conditioned media. The murine Rspo1 protein from this cell line has been used extensively to lower the cost of organoid culture and to maintain Lgr5+ stem cells, while the FC and HA tags make it easy to purify or characterize.
Trevigen, Inc. is a rapidly growing biotechnology company focused on the development of products and technology for cancer research, emphasizing apoptosis, DNA damage and repair, and cancer cell function and behavior.